Precision diagnostics in paediatric dermatology: Advancing management of tinea capitis through dermatophyte PCR

• DOI: 10.1111/jdv.20147
• Publication Date: 2024-05-31T12:29:11Z
• Authors: M. Theiler; I. Luchsinger; A. C. Rast; A. Schwieger‐Briel; L. Weibel; P. P. Bosshard
• Keywords: Dermatophyte; Tinea Capitis; Direct examination

Abstract Background Tinea capitis (TC) is the most frequent dermatophyte infection in children requiring systemic and topical treatment for several weeks. Traditionally, diagnosis monitoring were based on microscopic examination fungal culture of scales plucked hairs, which both have significant limitations. Objectives To investigate role polymerase chain reaction (PCR) TC. Methods Scales hairs with TC investigated by PCR, at baseline during antifungal treatment. Results Seventeen included. At baseline, sensitivity PCR was 100% as compared to 60% 87% direct microscopy culture, respectively. Species identification consistent all cases. During follow‐up, analysis 38 samples under showed a 68%, 26% 89% while specificity 84%, 100%, therapy proved be false‐negative six false‐positive three instances. The latter turned negative after 4 weeks without further Conclusions Dermatophyte an excellent tool diagnostics providing rapid accurate results. Our findings suggest that due fast reliable results, it may replace confirm or exclude children. In course, diagnostic accuracy performance seem reduced limiting its value monitoring. Mycological cure ascertained should currently remain therapeutic goal.