Mycamoeba gemmipara nov. gen., nov. sp., the First Cultured Member of the Environmental Dermamoebidae Clade LKM74 and its Unusual Life Cycle

• DOI: 10.1111/jeu.12357
• Publication Date: 2016-08-20T07:32:45Z
• Authors: Quentin Blandenier; Christophe V. W. ...; David Singer; Michèle Vlimant; Anaële Simon; Clément Duckert; Enrique Lara
• Keywords: Environmental DNA

Abstract Since the first environmental DNA surveys, entire groups of sequences called “environmental clades” did not have any cultured representative. LKM74 is an amoebozoan clade affiliated to Dermamoebidae, whose presence pervasively reported in soil and freshwater. We obtained isolate from that we assigned by molecular phylogeny, close related freshwater clones. described Mycamoeba gemmipara based on observations made with light‐ transmission electron microscopy. It extremely small amoeba typical lingulate shape. Unlike other it lacked ornamentation its cell membrane, condensed chromatin formed characteristic patterns nucleus. M. displayed a unique life cycle: trophozoites walled coccoid stages which grew through successive buddings developed into branched structures holding cysts. These structures, measuring hundreds micrometres, are built as exclusive product osmotrophic feeding. To demonstrate genuine inhabitant, screened diversity survey performed experimental setup where pig cadavers were left decompose soils follow changes eukaryotic communities. was present all samples, although reads uncommon underneath cadaver.