Kinesin‐14 is Important for Chromosome Segregation During Mitosis and Meiosis in the Ciliate Tetrahymena thermophila

• DOI: 10.1111/jeu.12366
• Publication Date: 2016-09-06T06:06:42Z
• Authors: Yasuharu Kushida; Masak Takaine; Kentaro Nakano; Toshiro Sugai; Krishna Kumar Vas...; Mayukh Guha; Yu‐Yang Jiang; Jacek Gaertig; Osamu Numata
• Keywords: Macronucleus; Spindle pole body; Astral microtubules

Ciliates such as Tetrahymena thermophila have two distinct nuclei within one cell: the micronucleus that undergoes mitosis and meiosis macronucleus amitosis, a type of nuclear division does not involve bipolar spindle, but still relies on intranuclear microtubules. provide an opportunity for discovery factors specifically contribute to chromosome segregation based by identification affect micronuclear macronuclear division. Kinesin-14 is conserved minus-end directed microtubule motor cross-links microtubules contributes spindle sizing organization. Here, we use homologous DNA recombination knock out genes encode kinesin-14 orthologues (KIN141, KIN142) in Tetrahymena. A loss KIN141 led severe defects during both did amitosis. altered shape meiotic way consistent with KIN141's contribution organization poles. EGFP-tagged preferentially accumulated at poles prophase metaphase I. Thus, ciliates, important divisions spindle.