New parameters for in vitro development of cell lines of the species Astyanax bimaculatus (Linnaeus, 1758) and Geophagus proximus (Castelnau, 1855)

• DOI: 10.1111/jfb.15731
• Publication Date: 2024-04-18T09:26:36Z
• Authors: Ivanete de Olivei...; Lygia S. Nogueira; Rodrigo Petry Cor...; Glaucia Caroline ...; Diovanna Mirella ...; Allan Costa‐Malaq...; Edivaldo H. C. de...
• Keywords: 0301 basic medicine; 03 medical and health sciences

Abstract Intending to compare in vitro cell growth different conditions, we established cultures using fin biopsies of two freshwater fishes, Astyanax bimaculatus and Geophagus proximus . Three culture media (Leibovitz–L‐15, Dulbecco's Modified Eagle Medium [DMEM], 199) were employed, with or without the addition AmnioMax, maintaining a standard temperature 29°C. Based on results obtained, standardized protocol which medium 199 was less efficient for both species. Notably, G. cells exhibited superior proliferation DMEM L‐15 media, whereas A. demonstrated better parameters exclusively medium. Successful subculturing good index observed, accompanied by preserved morphological characteristics. Therefore, methodology outlined this study represents an advancement establishing fish cultures.