Erythritol modulates the polarization of macrophages: Potential role of tumor necrosis factor‐α and Akt pathway

• DOI: 10.1111/jfbc.13960
• Publication Date: 2021-12-19T17:04:40Z
• Authors: Hassan S. Alamri; Maaged A. Akiel; Talal S. Alghassab; Mohammad A. Alfhili; Bahauddeen M. Alr...; Maha Aljumaa; Tlili Barhoumi
• Keywords: Proinflammatory cytokine; Macrophage polarization; Erythritol; Pyroptosis; Mannose receptor; CD11c; CD163

Low-calorie sweeteners are substitutes for sugar and frequently used by patients with cardiometabolic diseases. Erythritol, a natural low-calorie alcohol, was linked to diseases in several recent metabolomics studies. However, the characterization of its role disease development is lacking. Macrophage polarization orchestrates immune response various inflammatory conditions, most notably disease. Therefore, physiological effects Erythritol on THP-1 macrophages were investigated. We observed an increased cellular abundance proinflammatory M1 macrophages, characterized CD11c, TNF-α, CD64, CD38, HLA-DR markers decreased anti-inflammatory M2 mannose receptor CD206. The, ROS generation, activation AKT pathway, cytosolic calcium overload, cell cycle arrest at G1 phase. Concomitantly, population necroptotic observed. In conclusion, we provide evidence that induced phenotype this associated macrophages. PRACTICAL APPLICATIONS: This assessment provides particularly THP-1-derived Our results support driving inflammation insights as inducer necroptosis derived could be