Gibberellic acid‐induced hepatorenal dysfunction and oxidative stress: Mitigation by quercetin through modulation of antioxidant, anti‐inflammatory, and antiapoptotic activities

• DOI: 10.1111/jfbc.14069
• Publication Date: 2022-01-05T09:20:50Z
• Authors: Mohamed M. Soliman; Ahmed Gaber; Walaa F. Alsanie; Wafaa A. Mohamed; Mohamed M. M. Met...; Abdelhadi A. Abde...; Mohamed Elbadawy; Mustafa Shukry
• Keywords: Malondialdehyde; Gibberellic acid; Liver function

The plant growth regulator gibberellic acid (GA3) is widely used in agriculture many countries. However, little known about its danger to human health or physiologic and biochemical pathways. Our study examined the effect of GA3 on liver kidney function quercetin hepatorenal toxicity induced by four groups male albino rats. For 4 weeks, control group (CNT) received saline, (QR) daily intraperitoneal injections (50 mg/kg/BW) dissolved (55 via oral gavage, protective was injected with gavaged same doses QR mg/kg/BW +GA3 55 mg/kg/BW). injury, as shown elevated serum glutamic pyruvic transaminase, oxaloacetic gamma-glutamyl transferase (GPT, GOT, GGT) well increased levels creatinine, urea, uric acid. Hepatorenal demonstrated a significant increase tissue malondialdehyde (MDA) decreased antioxidant enzyme activity, such catalase (CAT) superoxide dismutase (SOD), accompanied subsequent decrease glutathione peroxidase (GPx) GA3-treated Administration significantly protected against toxic through normalization hepatic renal markers. It also retrieved ability modulating at molecular level upregulation antiapoptotic genes downregulation transforming factor-β1 (TFG-β1), cyclooxygenase-2 (COX-2), nuclear factor-kappa B (NF-κB). Impairment confirmed histologic immunohistochemical analyses. Pretreatment effective attenuating histopathologic changes tissues regulating immunoexpression caspase-3 Bcl-2 return them more normal values. Practical applications dysfunction caused ameliorated administration. Moreover, potential reverse inflammatory properties, inhibiting production free radicals inflammation-associated cytokines, antioxidants activity.