Carp edema virus infection associated gill pathobiome: A case report

• DOI: 10.1111/jfd.13670
• Publication Date: 2022-06-16T08:55:08Z
• Authors: Miroslava Palíková; Ľubomír Pojezdal; Lenka Dávidová‐Ge...; Věra Nováková; Jiří Pikula; Ivana Papežíková; Hana Minářová; Ivana Mikulíková; Iva Dyková
• Keywords: Common carp

Abstract Understanding disease aetiology and pathologic mechanisms is essential for fish health evaluation. Carp edema virus (CEV) the causative agent of a (CEVD) responsible high mortality rates in both wild cultured common carp Cyprinus carpio . Inspection two specimens from pond with revealed CEV infection host its ectoparasite ( Argulus foliaceus ). In addition to flavobacteria, well known be associated gill lesions, we found that free‐living eukaryotes (amoebae ciliates) temporary parasite Ichthyobodo spp.) colonizing gills may also contribute alterations structure and/or function, either directly, through firm ) or weak (amoebae) attachment trophozoites epithelium, indirectly, carriage pathogenic bacteria. Bacterial assemblages rich families genera, predominance Cetobacterium spp. low‐intensity alteration tissue Flavobacterium extensive necrotic were detected within cytoplasm amoebae using high‐throughput sequencing. Quantitative PCR indicated F swingsii as prevailing flavobacterial species less affected psychrophilum extensively gills. This case study suggests eukaryotic organisms part pathobiome irreversible lesions seen CEVD. Emphasizing complexity mutual relationships between bacterial co‐pathogens, further studies regarding factors trigger pathology influence severity CEV‐positive are needed.