Chitin extraction from shrimp wastes by single step fermentation with Lactobacillus acidophilus FTDC3871 using response surface methodology

• DOI: 10.1111/jfpp.14895
• Publication Date: 2020-08-28T03:53:12Z
• Authors: Joo Shun Tan; Sahar Abbasiliasi; Chee Keong Lee; Pongsathon Phapug...
• Keywords: Central composite design

This study aimed to produce chitin and chitosan from shrimp wastes using lactic acid bacteria (LAB). From four strains of LAB their mixture, Lactobacillus acidophilus FTDC3871 was selected due higher proliferation production. The fermentation parameters influencing on yields such as glucose concentration (w/w), inoculum size (v/w), time (h), water content (mL) were optimized response surface methodology (RSM) with central composite design (CCD). optimum levels for concentration, 15% 5% 100 ml after 72 hr incubation at 37°C. These conditions led 90.8% demineralization 76% deproteinization which resulted in 57.7% yield. highest concentrations carotenoid extracted accumulated hydrolysate 183.9 μg/g 21.3 g/L, respectively. Results showed potential application L. extraction waste. Practical applications In this study, fermenting (LAB) explored solve the problems conventional purification processes. method seems promising, since protein, chitin, could be obtained simultaneously eco-friendly. Since efficiency process depends factors strains, size, carbon source initial pH evolution during fermentation, duration, therefore, a suitable strain improve by optimizing above influence statistical approach, (RSM).