Long noncoding RNA TMPO‐AS1/miR‐126‐5p/BRCC3 axis accelerates gastric cancer progression and angiogenesis via activating PI3K/Akt/mTOR pathway

• DOI: 10.1111/jgh.15362
• Publication Date: 2020-12-10T05:33:42Z
• Authors: Yu Hu; Ying Zhang; Meng Ding; Ruisi Xu
• Keywords: Competing Endogenous RNA

Gastric cancer (GC) is an aggressive tumor featured by uncontrolled cell proliferation and metastasis. In recent years, long noncoding RNAs (lncRNAs) act as crucial regulators biological markers in multiple cancers. LncRNA TMPO-AS1 has been revealed to be oncogene some Nevertheless, there little known about the role of GC.Reverse transcription-quantitative polymerase chain reaction analysis was used examine expression level GC tissues cells. Cell Counting Kit-8, colony formation, wound healing assays, western blot were performed determine RNA pull-down, luciferase reporter, immunoprecipitation assays test interaction among TMPO-AS1, miR-126-5p, BRCC3.TMPO-AS1 highly expressed Upregulated closely associated with adverse prognosis patients. Functional showed that promoted proliferation, migration, angiogenesis. Furthermore, it found acted a competing endogenous for miR-126-5p upregulate BRCC3 expression. Rescue facilitated cellular progression sponging upregulating BRCC3. addition, we effects TMPO-AS1/miR-126-5p/BRCC3 axis on related PI3K/Akt/mTOR pathway.Our study demonstrated involved via regulation pathway, which might provide potential therapeutic strategy GC.