AtSec62 is critical for plant development and is involved in ER‐phagy in Arabidopsis thaliana

• DOI: 10.1111/jipb.12872
• Publication Date: 2019-09-04T14:26:26Z
• Authors: Shuai Hu; Hao Ye; Yong Cui; Liwen Jiang
• Keywords: Tunicamycin; Autophagosome

Abstract The endoplasmic reticulum (ER) is the major site for protein folding in eukaryotic cells. ER homeostasis essential development of an organism, whereby unfolded response (UPR) within precisely regulated. ER‐phagy a newly identified selective autophagic pathway removal misfolded or proteins mammalian Sec62, component translocon complex, was recently characterized as receptor during stress recovery phase mammals. In this study, we demonstrated that Arabidopsis Sec62 (AtSec62) required plant and might function plants. We showed AtSec62 ER‐localized membrane with three transmembrane domains (TMDs) its C‐terminus facing to lumen. because atsec62 mutants display impaired vegetative growth, abnormal pollen decreased fertility. are sensitive towards tunicamycin (TM)‐induced stress, whereas overexpression subsequently enhances tolerance phase. Moreover, YFP‐AtSec62 colocalizes autophagosome marker mCh‐Atg8e ring‐like structures upon induction. Taken together, these data provide evidence pivotal roles ER‐phagy.