Oryza sativa mediator subunit OsMED25 interacts with OsBZR1 to regulate brassinosteroid signaling and plant architecture in rice

• DOI: 10.1111/jipb.12914
• Publication Date: 2020-01-28T12:26:51Z
• Authors: Yuekun Ren; Xiaojie Tian; Shuyu Li; Enyang Mei; Mingliang He; Jiaqi Tang; Min Xu; Xiufeng Li; Zhenyu Wang; Chuanyou Li; Qingyun Bu
• Keywords: Brassinosteroid; Mediator; Corepressor; Transcription

Abstract Brassinosteroids (BRs) are plant‐specific steroid hormones which regulate plant growth, development, and adaptation. Transcriptional regulation plays key roles in hormone signaling. A mediator can serve as a bridge between gene‐specific transcription factors the RNA polymerase machinery, functioning an essential component regulating transcriptional process. However, whether is involved BR signaling unknown. Here, we discovered that Oryza sativa subunit 25 (OsMED25) important regulator of rice Phenotypic analyses showed OsMED25‐RNAi osmed25 mutant presented erect leaves, observed BR‐deficient mutants. In addition, exhibited decreased sensitivity. Genetic analysis indicated could suppress enhanced phenotype Osbzr1‐D . Further biochemical OsMED25 interacts with OsBZR1 vivo , enriched on promoter target genes. sequencing affects expression approximately 45% OsBZR1‐regulated genes mainly functions corepressor OsBZR1. Together, these findings revealed regulates by interacting modulating genes, thus expanding our understanding mediators