ERF4 interacts with and antagonizes TCP15 in regulating endoreduplication and cell growth in Arabidopsis

• DOI: 10.1111/jipb.13323
• Publication Date: 2022-07-01T06:39:40Z
• Authors: An‐Ming Ding; Chuan‐Tao Xu; Qiang Xie; Ming‐Jin Zhang; Ning Yan; Chang‐Bo Dai; Jing Lv; Meng‐Meng Cui; Wei‐Feng Wang; Yu‐He Sun
• Keywords: Endoreduplication; E2F

Endoreduplication is prevalent during plant growth and development, often correlated with large cell organ size. Despite its prevalence, the transcriptional regulatory mechanisms underlying transition from mitotic division to endoreduplication remain elusive. Here, we characterize ETHYLENE-RESPONSIVE ELEMENT BINDING FACTOR 4 (ERF4) as a positive regulator of through function repressor. ERF4 was specifically expressed in mature tissues which cells were undergoing expansion, but rarely young organs. Plants overexpressing exhibited much larger organs, while plants that lacked functional displayed smaller organs than wild-type. further shown regulate size by controlling endopolyploidy level nuclei. Moreover, physically associates class I TEOSINTE BRANCHED 1/CYCLOIDEA/PCF (TCP) protein TCP15, transcription factor inhibits activating expression key cell-cycle gene, CYCLIN A2;3 (CYCA2;3). A molecular genetic analysis revealed promotes directly suppressing CYCA2;3. Together, this study demonstrates TCP15 module antagonistically each other's activity regulating downstream genes, thereby switch cycle leaf development. These findings expand our understanding how control fine-tuned an ERF4-TCP15 complex.