Rice FLOURY ENDOSPERM22, encoding a pentatricopeptide repeat protein, is involved in both mitochondrial RNA splicing and editing and is crucial for endosperm development

• DOI: 10.1111/jipb.13402
• Publication Date: 2022-11-05T06:09:01Z
• Authors: Hang Yang; Yunlong Wang; Yunlu Tian; Xuan Teng; Zehui Lv; Jie Lei; Erchao Duan; Hui Dong; Xue Yang; Yuanyan Zhang; Yinglun Sun; Xiaoli Chen; Xiuhao Bao; Rongbo Chen; Chuanwei Gu; Yipeng Zhang; Xiaokang Jiang; Wenyu Ma; Pengcheng Zhang; Yi Ji; Yu Zhang; Yihua Wang; Jianmin Wan
• Keywords: Pentatricopeptide repeat

Most of the reported P-type pentatricopeptide repeat (PPR) proteins play roles in organelle RNA stabilization and splicing. However, PPRs involved both splicing editing have rarely been reported, their underlying mechanism remains largely unknown. Here, we report a rice floury endosperm22 (flo22) mutant with delayed amyloplast development endosperm cells. Map-based cloning complementation tests demonstrated that FLO22 encodes mitochondrion-localized PPR protein. Mutation resulting defective trans-splicing mitochondrial nad1 intron 1 perhaps causing instability mature transcripts affected assembly activity complex Ⅰ, morphology function. RNA-seq analysis showed expression levels many genes starch sucrose metabolism were significantly down-regulated flo22 compared wild type, whereas related to oxidative phosphorylation tricarboxylic acid cycle up-regulated. In addition involvement as protein, found interacted DYW3, DYW-type they may function synergistically editing. The present work indicated plays an important role plant growth by participating maturation multi-site messager RNA.