Histone deacetylase OsHDA706 increases salt tolerance via H4K5/K8 deacetylation of OsPP2C49 in rice

• DOI: 10.1111/jipb.13470
• Publication Date: 2023-02-21T16:24:03Z
• Authors: Kai Liu; Jijin Chen; Shang Sun; Xu Chen; Xinru Zhao; Yingying Hu; Guoxiao Qi; Xiya Li; Bo Xu; Jun Miao; Chao Xue; Yong Zhou; Zhiyun Gong
• Keywords: Histone acetyltransferase; Chromatin immunoprecipitation; Histone H4; Histone deacetylase 5

ABSTRACT High salt is a major environmental factor that threatens plant growth and development. Increasing evidence indicates histone acetylation involved in responses to various abiotic stress; however, the underlying epigenetic regulatory mechanisms remain poorly understood. In this study, we revealed deacetylase OsHDA706 epigenetically regulates expression of stress response genes rice ( Oryza sativa L.). localizes nucleus cytoplasm significantly induced under stress. Moreover, oshda706 mutants showed higher sensitivity than wild‐type. vivo vitro enzymatic activity assays demonstrated specifically deacetylation lysines 5 8 on H4 (H4K5 H4K8). By combining chromatin immunoprecipitation mRNA sequencing, identified clade A protein phosphatase 2 C gene, OsPP2C49 , which as direct target H4K5 H4K8 acetylation. We found mutant Furthermore, knockout enhances tolerance stress, while its overexpression has opposite effect. Taken together, our results indicate OsHDA706, deacetylase, participates by regulating via deacetylation.