The Gray Institute ‘open’ high‐content, fluorescence lifetime microscopes

• DOI: 10.1111/jmi.12057
• Publication Date: 2013-06-12T12:59:06Z
• Authors: P.R. BARBER; I.D.C. TULLIS; G.P. PIERCE; R.G. NEWMAN; J. PRENTICE; M.I. ROWLEY; D.R. MATTHEWS; S.M. AMEER‐BEG; B. VOJNOVIC
• Keywords: High-Content Screening

Summary We describe a microscopy design methodology and details of microscopes built to this ‘open’ approach. These demonstrate the first implementation time‐domain fluorescence in flexible automated platform with ability ease transition other advanced techniques from development use routine biology applications. This approach allows easy expansion modification capabilities, as it moves away commercial, monolithic, microscope body small, commercial off‐the‐shelf custom made modular components. Drawings diagrams our have been available under an open license for noncommercial at http://users.ox.ac.uk/~atdgroup . Several high‐content implementations constructed framework optimized specific applications multiwell plates tissue microarrays. In particular, three platforms incorporate FLIM via time‐correlated single photon counting fashion. also present data experiments performed on these highlighting their wide‐field laser scanning capabilities designed microscopy. Devices using designs form radiation‐beam ‘end‐stations’ Oxford Surrey Universities, showing versatility extendibility