Living on the edge: the role of Atgolgin‐84A at the plant ER–Golgi interface

• DOI: 10.1111/jmi.12946
• Publication Date: 2020-07-23T05:36:34Z
• Authors: V. VIEIRA; C. PAIN; S. WOJCIK; T. SPATOLA ROSSI; J. DENECKE; A. OSTERRIEDER; C. HAWES; V. KRIECHBAUMER
• Keywords: COPII; COPI; Brefeldin A; Transport protein; Golgi membrane

The plant Golgi apparatus is responsible for the processing of proteins received from endoplasmic reticulum (ER) and their distribution to multiple destinations within cell. matrix components, such as golgins, have been identified suggested function putative tethering factors mediate physical connections between bodies ER network. Golgins are anchored membrane by C-terminus either through transmembrane domains or interaction with small regulatory GTPases. golgin N-terminus contains long coiled-coil domains, which consist a number α-helices wrapped around each other form structure similar rope being made several strands, reaching into cytoplasm. In animal cells, golgins also implicated in specific recognition cargo at Golgi.Here, we investigate Atgolgin-84A its subcellular localization potential role factor ER-Golgi interface. For this, fluorescent fusions an truncation lacking (Atgolgin-84AΔ1-557) were transiently expressed tobacco leaf epidermal cells imaged using high-resolution confocal microscopy. We show that localizes pre-cis-Golgi compartment labelled one COPII well tether protein AtCASP. Upon overexpression deletion mutant, transport impaired redirected vacuole. LAY DESCRIPTION: specialised found mammalian cells. It post office cell packages boxes destination many separate Specialised called ER. Here exact body fused jellyfish producing this combination This allows us see laser localises When produced high amounts cell, inhibited