Functional organisation of the endomembrane network in the digestive gland of the Venus flytrap: revisiting an old story with a new microscopy toolbox

• DOI: 10.1111/jmi.12957
• Publication Date: 2020-08-26T18:28:25Z
• Authors: C. BOULOGNE; C. GILLET; L. HUGHES; R. LE BARS; A. CANETTE; C.R. HAWES; B. SATIAT‐JEUNEMA...
• Keywords: Endomembrane system

Summary Up‐to‐date imaging approaches were used to address the spatiotemporal organisation of endomembrane system in secretory cells Dionaea muscipula . Different ‘slice and view’ methodologies performed on resin‐embedded samples finally achieve a 3D reconstruction cell architecture, using ultrastructural tomography, array serial block face‐scanning electron microscopy (SBF‐SEM), correlation, volume rendering at light level. Observations cryo‐fixed by high‐pressure freezing revealed changes that occur after trap activation prey digestion. They provide evidence for an original strategy adapts machinery specific unique case stimulated exocytosis plant cells. A first secretion peak is part rapid response deliver digestive fluids surface, which delivers needed stock materials ‘on site’. The second activity could then be associated with Golgi apparatus (GA), endoplasmic reticulum (ER) vacuolar machinery, order prepare subsequent round capture. Tubular continuum between ER stacks observed ZIO‐impregnated tissues may correspond efficient transfer mechanism lipids and/or proteins, especially use rapidly resetting molecular GA machinery. occurrence one permit continuous adjustment homeostasy. subcellular features outline key innovations compartmentalisation are cope needs such as full protein factory, ability create reservoirs periplasmic space. Shape‐derived forces pleiomorphic vacuole act signals accompany sorting entering flows cell.