Novel synthetic sulfoglycolipidIG20 facilitates exocytosis in chromaffin cells through the regulation of sodium channels

• DOI: 10.1111/jnc.13357
• Publication Date: 2015-09-14T09:11:04Z
• Authors: Andrea Crespo‐Cas...; Eva Punzón; Ricardo de Pascual; Marcos Maroto; Juan Fernando Padín; Isabel García‐Álv...; Carmen Nanclares; Lucía Ruiz‐Pascual; Luis Gandía; Alfonso Fernández...; Antonio G. García
• Keywords: Chromaffin cell

In search of druggable synthetic lipids that function as potential modulators synaptic transmission and plasticity, we synthesized sulfoglycolipid IG20, which stimulates neuritic outgrowth. Here, have explored its effects on ion channels exocytosis in bovine chromaffin cells. IG20 augmented the rate basal catecholamine release. Such effect did not depend Ca(2+) mobilization from intracellular stores; rather, IG20-elicited secretion entirely dependent entry through L-subtype voltage-activated channels. Those were recruited by cell depolarization mediated likely ability to enhance recruitment Na(+) at more hyperpolarizing potentials. Confocal imaging with fluorescent derivative IG20-NBD revealed rapid incorporation confinement into plasmalemma, supporting idea are exerted a plasmalemmal-delimited mechanism. Thus, seems mimic several physiological endogenous such regulation channels, signaling, exocytosis. Therefore, may become pharmacological tool for investigating role lipid environment neuronal excitability, neurotransmitter release, efficacy, plasticity. It also inspire synthesis sulfoglycolipids aimed increasing plasticity efficacy neurodegenerative diseases traumatic brain-spinal cord injury. The novel mimics This profile eventually drive enhanced efficacy.