FABP‐1 gene ablation impacts brain endocannabinoid system in male mice

• DOI: 10.1111/jnc.13664
• Publication Date: 2016-05-11T18:11:01Z
• Authors: Gregory G. Martin; Sarah Chung; Danilo Landrock; Kerstin K. Landrock; Huan Huang; Lawrence J. Dangott; Xiaoxue Peng; Martin Kaczocha; Drew R. Seeger; Eric J. Murphy; Mikhail Y. Golovko; Ann B. Kier; Friedhelm Schroeder
• Keywords: Anandamide

Liver fatty acid-binding protein (FABP1, L-FABP) has high affinity for and enhances uptake of arachidonic acid (ARA, C20:4, n-6) which, when esterified to phospholipids, is the requisite precursor synthesis endocannabinoids (EC) such as arachidonoylethanolamide (AEA) 2-arachidonoylglycerol (2-AG). The brain derives most its ARA from plasma, taking up transporting it intracellularly via cytosolic proteins (FABPs 3,5, 7) localized within brain. In contrast, much more prevalent FABP1 not detectable in but instead highly expressed liver. Therefore, possibility that outside central nervous system may regulate AEA 2-AG was examined wild-type (WT) null (LKO) male mice. LKO increased levels AA-containing EC (AEA, 2-AG), correlating with free total serum. also non-ARA contain potentiating (EC*) oleoyl ethanolamide (OEA), PEA, 2-OG, 2-PG. Concomitantly, decreased serum ARA-containing EC, endocannabinoids. did elicit these changes EC* a result compensatory up-regulation enzymes (NAPEPLD, DAGLα) or chaperone 3, 5, 7, SCP-2, HSP70), cannabinoid receptors (CB1, TRVP1). These data show first time non-CNS markedly affected both 2-AG) well their Fatty protein-1 (FABP-1) (2-AG) FABP-1 acid-containing Read Editorial Highlight this article on page 371.