Evaluation of dermal extracellular matrix and epidermal–dermal junction modifications using matrix‐assisted laser desorption/ionization mass spectrometric imaging, in vivo reflectance confocal microscopy, echography, and histology: effect of age and peptide applications

• DOI: 10.1111/jocd.12135
• Publication Date: 2015-03-27T22:15:22Z
• Authors: Philippe Mondon; Mélanie Hillion; Olivier Peschard; Nada Andre; Thibault Marchand; Emmanuel Doridot; Marc GJ Feuilloley; Cédric Pionneau; Solenne Chardonnet
• Keywords: Mass spectrometry imaging

Summary This study was conducted to establish a new methodology for evaluating elements of dermal extracellular matrix ( ECM ), epidermal–dermal junction EDJ and effects molecules which can modulate their synthesis. is based on matrix‐assisted laser desorption/ionization mass spectrometric imaging MALDI ‐ MSI ). In vivo reflectance confocal microscopy in RCM ) echography were also used. Using immunohistochemistry methods explants, age‐related modification data obtained selected proteins (collagen I, collagen IV , VII XVII nidogen decorin/decorunt) used as reference studies. A developed with map epidermis dermis proteins. Then age modifications. high‐frequency ultrasounds evaluate undulation modifications caused by aging. Anti‐aging molecule evaluations performed blend palmitoyl oligopeptide tetrapeptide‐7. Immunohistochemistry studies demonstrated that the found be less abundant aged group explants vs . young except decorin. correlated results measurements indicated decrease depth upper part dermis. Echography peptide reduced subepidermal low‐echogenic band thickness improved its density. peptides structure placebo. preliminary raised some technical difficulties overcome. Further will identify more demonstrate interest this method cosmetic evaluations.