Effect of platelet‐rich plasma on proliferation and migration in human dermal fibroblasts

• DOI: 10.1111/jocd.12780
• Publication Date: 2018-10-03T00:50:31Z
• Authors: Eun Byul Cho; Gil Soon Park; Seok Soon Park; Ye Ji Jang; Kwang Ho Kim; Kwang Joong Kim; Eun Joo Park
• Keywords: Dermal fibroblast

Summary Background Platelet‐rich plasma (PRP) is a blood fraction that contains high concentrations of several growth factors. PRP has been recently used in skin wound healing and rejuvenation. However, the precise molecular mechanisms underlying PRP‐induced are unknown. Aims This study aimed to evaluate effects on extracellular matrix remodeling, which requires activation dermal fibroblasts. Methods Cell proliferation migration assay, enzyme‐linked immunosorbent analysis, Western blotting were performed PRP‐treated human Results Platelet numbers enhanced by 4.6‐fold compared whole blood. stimulated fibroblasts increased expression procollagen I alpha 1, elastin, MMP‐1, MMP‐2 also showed dramatic reduction phosphorylation c‐Jun N‐terminal kinase (JNK), whereas total JNK levels not significantly reduced. Conclusions Collectively, induced type collagen, MMP‐2, thereby accelerating healing. Our findings reveal basic PRP‐mediated tissue remodeling. Thus, these results could be exploited for clinical dermatology