The effect of allogenic human Wharton's jelly stem cells seeded onto acellular dermal matrix in healing of rat burn wounds

• DOI: 10.1111/jocd.13109
• Publication Date: 2019-09-26T04:34:07Z
• Authors: Mehra Nazempour; Davood Mehrabani; Rouhollah Mehdina...; Seyedeh‐Sara Hashemi; Amin Derakhshanfar; Shahrokh Zare; Mitra Zardosht; Javad Moayedi; Mahjoob Vahedi
• Keywords: Wharton's jelly; Silver sulfadiazine

Abstract Background Various methods were introduced to overcome the autograft shortage in burn wound care, including cell transplantation and tissue engineering. Aims To evaluate healing effect of allogenic human Wharton's jelly stem cells (hWJSCs) seeded onto acellular dermal matrix (ADM) rat injuries. Patients Methods Human provided from umbilical cord characterized before transplantation, growth kinetic was determined. Skin samples cosmetic surgeries used for preparation ADM. Forty male Sprague Dawley rats randomly divided into 4 equal groups. Third‐degree induced all animals by exposing hot water using a 2 cm ring 10 seconds. Group 1 burned that did not receive any treatment. After injury, second group received silver sulfadiazine (SSD), third treated just ADM, fourth × 6 hWJSCs The euthanized histologic evaluation after 7, 14, 21 days. Results be spindle shape positive osteogenic adipogenic induction mesenchymal markers but lacked hematopoietic markers. Population doubling time (PDT) 40.1 hours with an increasing trend until day 6th. Macro‐ microscopically, mild ADM moderate + Conclusion Allogenic improved process wounds denoting their therapeutic anti‐inflammatory effects can added literature.