A comparison of high‐risk human papillomavirus DNA detection between urine and cervical sample testing in women with abnormal Pap smears

• DOI: 10.1111/jog.15095
• Publication Date: 2021-11-09T02:04:06Z
• Authors: Awassada Punyasht...; Navin Horthongkham; Atthapon Jaishuen; Nida Jareemit; Vuthinun Achariya...
• Keywords: Vaginal Smears; Papillomavirus Infections; Uterine Cervical Neoplasms; Alphapapillomavirus; Thailand; Uterine Cervical Dysplasia; Sensitivity and Specificity; 6. Clean water; 3. Good health; 03 medical and health sciences; Cross-Sectional Studies; 0302 clinical medicine; DNA, Viral; Humans; Female; Prospective Studies; Papillomaviridae; Early Detection of Cancer; Papanicolaou Test

To compare the clinical performance of high-risk human papillomavirus (hrHPV) DNA detection between urine and cervical samples collected from same patient for CIN2+ lesions (high-grade squamous intraepithelial or cancer lesions). The secondary objectives were to evaluate agreement among hrHPV genotypes satisfaction sample collection.This prospective cross-sectional study enrolled 96 women with abnormal cytology who attended colposcopy clinic at Siriraj Hospital (Bangkok, Thailand) July 2016 January 2017. Self-collected random-voiding first stream into a universal sterile container immediately mixing preservative before pelvic examination. Cervical tissue sampling was performed according standard treatment guidelines. Both specimens sent extraction by Anyplex II HPV testing. Study patients surveyed collection.Carcinogenic positive rate 73% in 81% samples. sensitivity high both groups 86.2% 94.8%, respectively. Agreement 16 18 high, kappa values 0.86 subtypes 16/18. Urine specimen collection had significantly higher acceptability than collection.Urine testing real-time polymerase chain reaction demonstrated accuracy lesions, very good when compared