SPI1‐related protein inhibits cervical cancer cell progression and prevents macrophage cell migration

• DOI: 10.1111/jog.15336
• Publication Date: 2022-06-30T09:49:20Z
• Authors: Jing Jin; Xin Du; Limin Zhou; Dongmei Yao; Qian Zou
• Keywords: CCL22; HeLa; CCL25

The functions and molecular mechanisms of SPI1-related protein (SPIB) were examined in cervical cancer (CC) cells.Genes related to miscarriage prognosis CC identified by Kaplan-Meier differential expression analysis, respectively. Cell proliferation, apoptosis, migration, invasion cell counting kit-8, flow cytometry, transwell assays, potential SPIB speculated gene set enrichment analysis (GSEA) analysis. mRNA levels genes RT-qPCR western blot effect on macrophage cells was tested recruitment assay bioinformatics analysis.A total 753 dysregulated 88 TCGA samples with a history one or more miscarriages versus 208 no history. Also, 91 identified. SPIB, both prognosis, inhibited Hela invasion, facilitated apoptosis. GSEA disclosed that might play vital roles immunity, chemokine signaling pathway, chemotaxis/activation CC. Moreover, C-X-C motif ligand 8 (CXCL8), C-C 17 (CCL17), 25 (CCL25) cells, overexpression hampered THP-1 migration. Higher associated less M2 infiltration CC.SPIB CC-cell progression hindered migration