Microdissection testicular sperm extraction in male partner with non‐obstructive azoospermia: Fresh or frozen–thawed sperm for intracytoplasmic sperm injection?

• DOI: 10.1111/jog.16298
• Publication Date: 2025-04-25T03:24:34Z
• Authors: Sevinc Özmen; Esra Nur Tola
• Keywords: Testicular sperm extraction; Microdissection; Live birth; Sperm Retrieval; Assisted Reproductive Technology

Abstract Aim To compare the outcomes of intracytoplasmic sperm injection (ICSI) fresh and cryopreserved obtained via microdissection testicular extraction (micro‐TESE) in cases non‐obstructive azoospermia (NOA). Methods A total 147 patients with NOA who underwent micro‐TESE viable at Istanbul Medipol In Vitro Fertilization unit were recruited retrospectively. The divided into two groups depending on used (fresh or frozen–thawed) for ICSI: group ( n = 93) ICSI cycles spermatozoa micro‐TESE‐Thaw 54) spermatozoa. Patient demographics, embryo development, pregnancy compared between groups. Results No statistical difference was observed terms demographic features (age, body mass index, etc.) cycle characteristics (induction protocol, gonadotropin dose, etc.). rates quality also similar Higher clinical live birth than p <0.04 <0.003, respectively). miscarriage rate higher group, although did not reach significance. Conclusion(s) where frozen by are used, even if motile found after thawing, there may be a negative impact result. Fresh appear to result better males NOA.