Immunomodulatory/inflammatory effects of geopropolis produced by Melipona fasciculata Smith in combination with doxorubicin on THP-1 cells

• DOI: 10.1111/jphp.12649
• Publication Date: 2016-10-17T08:12:39Z
• Authors: Lucas Pires Garci...; Fernanda Lopes Conte; Eliza de Oliveira...; Bruno José Conti; Karina Basso Sant...; Marjorie de Assis...; Maria Teresa Cruz; José Maurício Sfo...
• Keywords: cell markers; Time Factors; Cell Survival; 610; immunomodulation; Monocytes; Propolis; 03 medical and health sciences; 0302 clinical medicine; Cell Line, Tumor; Antineoplastic Combined Chemotherapy Protocols; Animals; Humans; geopropolis; Inflammation; chemotherapeutic agent; Dose-Response Relationship, Drug; Interleukin-6; Macrophages; THP-1 cells; HLA-DR Antigens; Bees; Toll-Like Receptor 2; 3. Good health; Toll-Like Receptor 4; Doxorubicin; B7-1 Antigen; Inflammation Mediators; Biomarkers

Abstract

Objectives Geopropolis (GEO) in combination with doxorubicin (DOX) reduced HEp-2 cells viability compared to GEO and DOX alone. A possible effect of this combination on the innate immunity could take place, and its effects were analysed on THP-1 cell – a human leukaemia monocytic cell line used as a model to study monocyte activity and macrophage activity, assessing cell viability, expression of cell markers and cytokine production.

Methods THP-1 cells were incubated with GEO, DOX and their combination. Cell viability was assessed by MTT assay, cell markers expression by flow cytometry and cytokine production by ELISA.

Key findings GEO + DOX did not affect cell viability. GEO alone or in combination increased TLR-4 and CD80 but not HLA-DR and TLR-2 expression. GEO stimulated TNF-α production while DOX alone or in combination did not affect it. GEO alone or in combination inhibited IL-6 production.

Conclusions GEO exerted a pro-inflammatory profile by increasing TLR-4 and CD80 expression and TNF-α production, favouring the activation of the immune/inflammatory response. GEO + DOX did not affect cell viability and presented an immunomodulatory action. Lower concentrations of DOX combined to GEO could be used in cancer patients, avoiding side effects and benefiting from the biological properties of GEO.