Hypothesis: Trans‐splicing Generates Evolutionary Novelty in the Photosynthetic Amoeba Paulinella

• DOI: 10.1111/jpy.13247
• Publication Date: 2022-03-07T19:53:26Z
• Authors: Arwa Gabr; Timothy G. Stephens; Debashish Bhattac...
• Keywords: Trans-splicing; Lineage (genetic); Endosymbiosis

Plastid primary endosymbiosis has occurred twice, once in the Archaeplastida ancestor and Paulinella (Rhizaria) lineage. Both events precipitated massive evolutionary changes, including recruitment activation of genes that are horizontally acquired (HGT) redeployment existing pathways novel contexts. Here we address latter aspect micropora KR01 (hereafter, KR01) independently evolved spliced leader (SL) trans-splicing (SLTS) nuclear-derived transcripts. We investigated role this process gene regulation, origination, endosymbiont integration. Our analysis shows 20% give rise to transcripts with at least one (but some cases, multiple) sites SL addition. This process, which often occurs canonical cis-splicing acceptor (internal introns), results shorter may produce 5'-truncated proteins functions. SL-truncated fall into four categories show: (i) altered protein localization, (ii) function, structure, or (iii) loss valid alternative start codons, preventing translation, (iv) multiple addition 5'-terminus. The RNA required for SLTS putatively absent heterotrophic sister lineage photosynthetic species. Moreover, a high proportion derived from endosymbiotic transfer (EGT) HGT origin contain sequences. hypothesize truncation by facilitate generation expression variants have enhanced fixation foreign host genome lineages, playing key