The therapeutic role of baicalein in combating experimental periodontitis with diabetes via Nrf2 antioxidant signaling pathway

• DOI: 10.1111/jre.12722
• Publication Date: 2019-12-19T11:12:33Z
• Authors: Chunhui Zhu; Ying Zhao; Xiaoyan Wu; Cui Qiang; Jin Liu; Jianfeng Shi; Jianzhong Gou; Dandan Pei; Ang Li
• Keywords: Baicalein

Oxidative stress has been suggested as an important pathogenic factor contributing to chronic periodontitis with diabetes mellitus (CPDM). Previous studies have revealed the potential therapeutic properties of baicalein (BCI) in oxidative stress-related diseases; however, antioxidant effects BCI on therapy for individual CPDM remain largely unexplored. Nuclear erythroid 2-related 2 (Nrf2) plays a critical role cellular defence against stress. In this study, we aim determine whether prevents diabetes-related periodontal tissue destruction by regulating Nrf2 signaling pathway.Human gingival epithelial cells (hGECs) were challenged high glucose (HG, 25 mmol/L) and/or lipopolysaccharide (LPS, 20 µg/mL). Reactive oxygen species (ROS) detected fluorescence-activated cell sorting. The changes antioxidant-related genes, including Nrf2, catalase (Cat), glutamate-cysteine ligase catalytic subunit (Gclc), superoxide dismutase 1 (Sod1), and (Sod2), quantified real-time PCR. localization phospho-Nrf2 (pNrf2, S40) nucleus was immunofluorescence staining laser scanning confocal microscope (LSCM). PNrf2 total form determined using western blot. above indicators together mitochondrial membrane (MMP) further investigated hGECs pre-treated different concentrations (0.01, 0.1, or 0.5 µg/mL) before stimulated HG plus LPS (GP). Finally, activating pathway relieving alveolar bone absorption examined model Sprague Dawley rats. rats oral gavaged (50, 100, 200 mg/kg daily). pNrf2 immunohistochemistry, microcomputed tomography.Our results showed that ROS significantly increased both groups LPS, strongest generation GP group. terms ROS-related gene expression, found mRNA levels Cat, Gclc, Sod1, Sod2 decreased groups. consistent induction group, expression group compared those Also, exhibited same trend genes. However, loss induced abolished pre-treatment Interestingly, observed promoted translocation pNrf2, well its target genes (Cat, Sod2). animal model, (at concentrations: 50, mg/kg) markedly number pNrf2-positive mitigated loss.Our data clinic application under conditions, suggesting new drug patients.