Fifteen novel universal primer pairs for sequencing whole chloroplast genomes and a primer pair for nuclear ribosomal DNAs

• DOI: 10.1111/jse.12197
• Publication Date: 2016-02-26T08:51:20Z
• Authors: Ting Zhang; Chun‐Xia Zeng; Jun‐Bo Yang; Hong‐Tao Li; De‐Zhu Li
• Keywords: Primer (cosmetics)

Abstract Chloroplast genome information helps improve the phylogenetic resolution and can act as organelle‐scale barcodes in recently radiated plant groups. Previously we reported that nine universal primer pairs could amplify angiosperm whole chloroplast genomes by long‐range polymerase chain reaction using next‐generation sequencing. Although these primers show high universality efficiency for sequencing angiosperms, they did not fully resolve following two issues surrounding genomes: (i) approximately 30% of angiosperms cannot be amplified successfully; (ii) only fresh leaves applied. In this study, designed another set 15 amplifying to complement original pairs. Furthermore, a pair nuclear ribosomal DNAs (nrDNAs). To validate functionality primers, tested 44 species with silica gel‐dried have been shown The result showed that, 65.9% 88.6% leaves, nrDNAs amplified, respectively. addition, all leaf samples successfully amplified. comprise partial sequences 18S 26S, along complete sequence 5.8S internal transcribed spacers ITS1 ITS2. mean size nrDNA was 5800 bp. This study shows is an indispensable tool are important supplement