Circadian variation of metabotropic glutamate receptor 5 availability in the rat brain
circadian rhythm
Male
Positron emission tomography
Light
Pyridines
Receptor, Metabotropic Glutamate 5
Brain
Circadian Rhythm
Rats
Rats, Sprague-Dawley
Random Allocation
03 medical and health sciences
0302 clinical medicine
Positron-Emission Tomography
Oximes
Animals
rat
metabotropic glutamate receptor
[11C]ABP688
DOI:
10.1111/jsr.12432
Publication Date:
2016-06-30T05:26:44Z
AUTHORS (10)
ABSTRACT
SummaryThe metabotrophic subtype 5 glutamate receptor (mGluR5) plays a critical role in synaptic plasticity besides its involvement in numerous neurological disorders, such as depression. As mGluR5 availability in humans is altered in sleep deprivation, we hypothesized that mGluR5 availability underlies a circadian variation. To investigate whether mGluR5 underlies potential circadian changes we measured its density in a randomized fashion at six different daytimes in 11 adult Sprague–Dawley rats. mGluR5 density was quantified by positron emission tomography (PET) using the radioactive ligand [11C]ABP688. [11C]ABP688 uptake was quantified in nine regions of interest with a reference tissue model. Significant differences in the binding potential (BPND) and therefore mGluR5 availability between the different circadian times were found in cortex, cingulate cortex, amygdala, caudate putamen and nucleus accumbens. Further post‐hoc statistical analysis (Tukey–Kramer test) of the different time‐points revealed significant changes in BPND between 07:00 hours (start of light‐on phase) and 15:00 hours (last time‐point of the light‐on phase) in the caudate putamen. This study shows that mGluR5 availability is increased during the light‐on, or sleep phase, of rodents by approximately 10%. Given that altered mGluR5 densities play a role in psychiatric disorders, further investigation is warranted to evaluate their circadian involvement in mood changes in humans.
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