Platelet Activation in Cats with Hypertrophic Cardiomyopathy

• DOI: 10.1111/jvim.12325
• Publication Date: 2014-02-24T18:42:55Z
• Authors: F. Tablin; T. Schumacher; M. Pombo; C.T. Marion; K. Huang; J.W. Norris; K.E. Jandrey; M.D. Kittleson
• Keywords: P-selectin

Background Cats with hypertrophic cardiomyopathy ( HCM ) are at risk for development of systemic thromboembolic disease. However, the relationship between platelet activation state and cardiovascular parameters associated is not well described. Objectives To characterize by flow cytometric evaluation P‐selectin semiquantitative Western blot analysis soluble platelet‐endothelial cell adhesion molecule‐1 sPECAM ‐1). Animals Eight normal healthy cats (controls) owned staff students School Veterinary Medicine 36 from UC Davis Feline Research Laboratory were studied. Methods Platelet‐rich plasma PRP was used all cytometry studies. Platelet surface CD 41 expression evaluated before after ADP stimulation. ‐1 platelet‐poor that had been stabilized aprotinin. Standard echocardiographic studies performed. Results Resting platelets severe increased compared to controls, expressed higher density reflected their mean fluorescence intensities MFI ). Stimulation also resulted in significantly . Increased correlated presence a heart murmur end‐systolic cavity obliteration ESCO moderate above those control cats. Conclusions Clinical Importance may be useful biomarkers indicating HCM.