Interobserver Agreement Using Histological Scoring of the Canine Liver

• DOI: 10.1111/jvim.14684
• Publication Date: 2017-03-14T20:57:40Z
• Authors: J.A. Lidbury; A. Rodrigues Hoff...; R. Ivanek; J.M. Cullen; B.F. Porter; F. Oliveira; T.J. Van Winkle; G.C. Grinwis; J.S. Sucholdolski; J.M. Steiner
• Keywords: Histology; Cohen's kappa; Grading (engineering); Kappa

Background Grading schemes for the assessment of hepatic fibrosis and necroinflammatory activity in humans previously have been applied to dogs with chronic hepatitis. Interobserver agreement is a desirable characteristic any histological scoring scheme. Hypothesis/Objectives To assess interobserver associated pathologists using published scheme compare scores assigned serial sections stained hematoxylin & eosin (H&E) picrosirius red. Animals Histological liver from 50 variable degrees were selected institutional tissue archives. Methods Six board‐certified veterinary anatomic sections. The multiuser kappa statistic was calculated agreement. Fibrosis stage red H&E compared Wilcoxon signed‐rank test. Results Multiuser statistics H&E‐stained 0.35 0.16, respectively. There no difference median section ( P = .248). Conclusions Clinical Importance fair when assessed poor they activity. This suboptimal must be taken into account by clinicians making decisions based on histology reports design studies evaluating these findings. decrease this variability, ideally >1 pathologist should evaluate each section.