Long‐term impact of tylosin on fecal microbiota and fecal bile acids of healthy dogs

• DOI: 10.1111/jvim.15635
• Publication Date: 2019-11-01T05:43:41Z
• Authors: Alison C. Manchester; Craig B. Webb; Amanda B. Blake; Fatima Sarwar; Jonathan A. Lidbury; Jörg M. Steiner; Jan S. Suchodolski
• Keywords: Tylosin

Abstract Background Tylosin is commonly prescribed to dogs with diarrhea. Orally administered antibiotics may alter the intestinal microbiota, which responsible for crucial key bile acid (BA) biotransformation reactions. Objectives To prospectively evaluate impact of tylosin administration on fecal microbiota and unconjugated acids (UBAs) over time. Animals Sixteen healthy adult dogs. Methods Prospective, randomized controlled clinical trial. Dogs were receive 20 mg/kg or a placebo capsule PO q12h 7 days while undergoing daily scoring. Fecal samples collected 0, 7, 21, 63. The was assessed using quantitative PCR 16S rRNA gene sequencing. Unconjugated BAs gas chromatography‐mass spectrometry (GC‐MS). Results scores unchanged during administration. In group, no significant changes observed in UBA concentrations. Day from tylosin‐exposed exhibited decreased bacterial diversity (observed species, Chao1, Shannon, P < .001) characterized by decreases anaerobes Fusobacteriaceae (linear discriminant analysis [LDA] score, 5.03) Veillonellaceae (LDA 4.85). Primary concentrations increased at day 21 (median, [range]; 7.42, [0.67‐18.77] μg/kg; = .04) 63 (3.49 [0‐28.43] .02) compared 0 (.14 [.03‐1.19] μg/kg) receiving tylosin. At 63, taxa not significantly different but extent microbial recovery individualized. Conclusions Clinical Importance causes dysbiosis corresponding shifts UBAs. Changes did uniformly resolve after discontinuation