Prevalence of positive urine culture in the presence of inactive urine sediment in 1049 urine samples from dogs

• DOI: 10.1111/jvim.16378
• Publication Date: 2022-02-02T18:39:51Z
• Authors: Nicole A. Strachan; Erin N. Hales; Julie R. Fischer
• Keywords: Veterinary medicine; 04 agricultural and veterinary sciences; Urinalysis; Sensitivity and Specificity; 6. Clean water; 3. Good health; 0403 veterinary science; pyuria; Dogs; SF600-1100; Urinary Tract Infections; Prevalence; Animals; bacterial cystitis; SMALL ANIMAL; Dog Diseases; proteinuria; urinary tract infection; Retrospective Studies

AbstractBackgroundUrinalysis (UA) is often used to screen for bacterial cystitis, regardless of sediment results, and followed up by quantitative urine culture (UC) for definitive diagnosis.ObjectivesDetermine prevalence of positive UCs in dogs with inactive urine sediments on routine UA.AnimalsA total of 1049 urine samples with inactive urine sediments and UCs collected from dogs presented to a veterinary specialty hospital between January 2018 and February 2020.MethodsRetrospective study of dogs with an inactive urine sediment on routine UA and follow‐up UCs. Signalment, UA findings, proteinuria, and UC results were recorded. Associations among these findings were assessed using multivariate logistic regression carried out using a backward stepwise method.ResultsOverall prevalence of positive UC was 3.4% (95% confidence interval [CI], 2.4‐4.8). Escherichia coli was the most commonly isolated bacteria. Only naturally voided samples were associated with increased prevalence of positive culture when compared to collection by cystocentesis or a non‐specified method. No statistically significant association with culture positivity was found for urine protein‐to‐creatinine ratio, urine specific gravity, urine pH, breed, age, or sex.Conclusions and Clinical ImportanceBased on the low prevalence (3.4%) of positive culture in urine samples from dogs with inactive sediment on routine UA and the relatively high cost of UC and sensitivity, cost‐benefit analysis including clinical suspicion of lower urinary tract disease should inform testing decisions, rather than routinely performing cultures on urine samples without active sediments.