Evaluating the possibility of using acetone-butanol-ethanol (ABE) fermentation wastewater for bacterial cellulose production by Gluconacetobacter xylinus

• DOI: 10.1111/lam.12396
• Publication Date: 2015-01-23T08:31:26Z
• Authors: C. Huang; X.-Y. Yang; L. Xiong; H.-J. Guo; J. Luo; B. Wang; H.-R. Zhang; X.-Q. Lin; X.-D. Chen
• Keywords: 570; Abe Fermentation Wastewater; Butanols; 02 engineering and technology; Wastewater; Gluconacetobacter Xylinus; Microbiology; 01 natural sciences; Bacterial Cellulose; 630; 12. Responsible consumption; CULTURE; Acetone; X-Ray Diffraction; Spectroscopy, Fourier Transform Infrared; Bio-polymer; Cellulose; ACETIC-ACID; Acetic Acid; Science & Technology; Xylose; Ethanol; Gluconacetobacter xylinus; ACETOBACTER-XYLINUM; Polymer Structure; Carbon; 6. Clean water; 0104 chemical sciences; Glucose; Biotechnology & Applied Microbiology; 13. Climate action; Fermentation; Butyric Acid; CARBON SOURCE; 0210 nano-technology; Life Sciences & Biomedicine

To reduce the cost of bacterial cellulose (BC) production, the possibility of using acetone-butanol-ethanol (ABE) fermentation wastewater with high COD value (18 050 mg l(-1) ) for BC production by Gluconacetobacter xylinus was evaluated. After 7 days of fermentation, the highest BC yield (1·34 g l(-1) ) was obtained. The carbon sources including sugars (glucose and xylose), organic acids (acetic acid and butyric acid) and alcohol compounds (ethanol and butanol) were utilized by G. xylinus simultaneously during fermentation. Although the COD decrease ratio (about 14·7%) was low, the highest BC yield on COD consumption (56·2%, g g(-1) ) was relatively high and the remaining wastewater could be used for further BC fermentation. Besides, the environment of ABE fermentation wastewater showed small influence on the BC structure by comparison with the BC products obtained in traditional HS medium using field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). Overall, ABE fermentation wastewater is one promising substrate for BC production.The possibility of using acetone-butanol-ethanol (ABE) fermentation wastewater for bacterial cellulose (BC) production by Gluconacetobacter xylinus was evaluated in this study. This is the first time that ABE fermentation wastewater was used as substrate for BC fermentation. The results provide detail information of metabolism of G. xylinus in ABE fermentation wastewater and the influence of wastewater environment on the structure of BC samples. Overall, this bioconversion could reduce the cost of BC production greatly.