Development of cross-priming amplification for direct detection of the African Swine Fever Virus, in pig and wild boar blood and sera samples

• DOI: 10.1111/lam.12569
• Publication Date: 2016-03-22T11:13:13Z
• Authors: M. Frączyk; G. Woźniakowski; A. Kowalczyk; K. Niemczuk; Z. Pejsak
• Keywords: African Swine Fever; Wild boar; Classical swine fever; Domestic pig

African swine fever (ASF) is considered a major threat to the production of pigs worldwide. The ASF aetiological agent, ASFV, sole member Asfivirus genus, belonging Asfarviridae family. An effective vaccine not currently available, thus only measures spread control include, reliable and fast diagnosis. Officially approved, diagnostic methods virus isolation, serological assays, including enzyme‐linked immunosorbent assay immunoperoxidase (IPT) different modifications polymerase chain reaction (PCR). This paper describes first development application cross‐priming amplification method (CPA) for direct detection genetic ASFV material, in blood sera from wild boars. specific DNA. study showed that CPA had equal sensitivity, comparison official, universal probe library (UPL) real‐time PCR reached 7·2 copies standard plasmid DNA, containing p72 gene fragment. was capable detecting DNA all examined samples, originating pigs; n = 10 boars; 76. obtained results were also confirmed by officially PCR. developed might be further used local county veterinary officers, hunters or pig farmers, preliminary (ASFV) among infected boars, one most important facets transmission eastern Europe. Cross‐priming has been developed, material It shown rapid, sensitive isothermal directly collected