Identification and measurement of staphylococcal enterotoxin M fromStaphylococcus aureusisolate associated with staphylococcal food poisoning

• DOI: 10.1111/lam.12751
• Publication Date: 2017-04-26T04:11:32Z
• Authors: Y. Zhao; A. Zhu; J. Tang; C. Tang; J. Chen
• Keywords: Polyclonal antibodies; Food poisoning; Protein A

Staphylococcus aureus produces a wide variety of staphylococcal enterotoxins (SEs, SEA to SEX), which are responsible for food poisoning. This study is aimed establish system detect enterotoxin M (SEM) protein in matrixes. In the present study, sem gene was characterized S. isolate H4 associated with The amino acid sequence deduced SEM same as that previously identified from 04-02981. Subsequently, mature expressed Escherichia coli BL21 (DE3) cells and purified Ni-NTA spin column. polyclonal monoclonal antibody against it were prepared. Using these antibodies, highly sensitive, specific sandwich enzyme-linked immunosorbent assay (ELISA) developed capable detecting milk, meat rice. Cross-reactivity SEB, SEI SEK this method insignificant. Quantification secretion vitro using novel capture ELISA revealed mainly secreted during transition exponential stationary phase. Furthermore, production screened by PCR system. results indicated there two SEM+ strains 19 isolates originating cold dishes humans suffering investigations make possible assess hygiene supervision near future.Staphylococcal (SE) main causative agents Unlike classical SEs (SEA SEE), relationship between newly (SEG SEX) poisoning has not been clearly elucidated. Recently, mild emetic potential demonstrated, might be However, currently no commercial kit available immunological detection it. Therefore, we assayed facilitates expression vivo. addition, would helpful addressing relative incidence future.