Heterotopic vascularization and functionalization of implantable bio engineered hepatic tissue alleviates liver injury in rats

• DOI: 10.1111/liv.14267
• Publication Date: 2019-10-01T06:27:21Z
• Authors: Yi Li; Qiong Wu; Zhen Yang; Yuting He; Chengxin Weng; Mengyu Gao; Bingqi Zhang; Yujia Wang; Li Li; Fei Chen; Hong Bu; Ji Bao
• Keywords: Surface Modification; Liver tissue; Biocompatible material

The challenge of using bioengineered liver lies in sustaining the quantity high-quality hepatocytes and vasculature for blood perfusion. We characterized heparinization a porcine decellularized scaffold (DLS) as carrier to support hepatocyte angiogenesis, thereby developing functional vascularized hepatic tissue useful treat injury.The DLS was obtained by removal cellular components then subjected end-point attachment technique. heparinized DLSs were recellularized with rat spheroids construct engineered tissue. heterotopically implanted omentum majus model injury induced carbon tetrachloride (CCl4 ).Hepatocyte remained viable at least 10 weeks vivo. entire populated arranged well. volume group expanded over 400-fold. Liver-specific functions such albumin synthesis, glycogen storage cytochrome P 3A4 activity highly expressed In addition, endothelial cells recruited, shown CD31 staining, new vessels formed, visualized fluorescein isothiocyanate-labelled dextran intravital confocal microscopy. alleviated CCl4 -induced regulating deposition degradation extracellular matrix.Primary survived an extended time on generate that can alleviate rats.