Combined analysis of gut microbiota, diet and PNPLA3 polymorphism in biopsy‐proven non‐alcoholic fatty liver disease

• DOI: 10.1111/liv.14899
• Publication Date: 2021-04-25T09:20:52Z
• Authors: Sonja Lang; Anna Martin; Xinlian Zhang; Fedja Farowski; Hilmar Wisplinghoff; Maria J.G.T. Vehr...; Marcin Krawczyk; Angela Nowag; Anne Kretzschmar; Claus Scholz; Philipp Kasper; Christoph Roderburg; Raphael Mohr; Frank Lammert; Frank Tacke; Bernd Schnabl; Tobias Goeser; Hans‐Michael Steffen; Münevver Demir
• Keywords: 0301 basic medicine; Biopsy; Clinical Sciences; Chronic Liver Disease and Cirrhosis; 610; microbiome; Clinical sciences; Polymorphism, Single Nucleotide; Oral and gastrointestinal; 03 medical and health sciences; Non-alcoholic Fatty Liver Disease; NAFLD; Genetics; microbiota; 2.1 Biological and endogenous factors; Humans; Polymorphism; Metabolic and endocrine; PNPLA3; Nutrition; Aged; 2. Zero hunger; Biomedical and Clinical Sciences; Gastroenterology & Hepatology; Liver Disease; Prevention; Diabetes; NASH; Membrane Proteins; Single Nucleotide; Lipase; Diet; Gastrointestinal Microbiome; 3. Good health; Good Health and Well Being; Cross-Sectional Studies; nutrition; Liver; Digestive Diseases; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

Non-alcoholic fatty liver disease (NAFLD) is a global health burden. Risk factors for severity include older age, increased body mass index (BMI), diabetes, genetic variants, dietary and gut microbiota alterations. However, the interdependence of these their individual impact on remain unknown.In this cross-sectional study, we performed 16S gene sequencing using fecal samples, collected intake, PNPLA3 variants clinical histology parameters in well-described cohort 180 NAFLD patients. Principal component analyses were used dimensionality reduction data. Simple multiple stepwise ordinal regression performed.Complete data available 57 In simple analysis, features associated with metabolic syndrome had highest importance regarding severity. BMI was most important factor fibrosis stage (OR per kg/m2 : 1.23, 95% CI 1.10-1.37, P < .001). The risk allele strongest association histological grade steatosis 5.32, 1.56-18.11, = .007), followed by specific patterns. Low abundances Faecalibacterium, Bacteroides Prevotella high Gemmiger degree inflammation, ballooning stages fibrosis, even after taking other cofactors into account.BMI but bacterial all different features, which underscore multifactorial pathogenesis NAFLD.