Carbohydrate‐binding agents act as potent trypanocidals that elicit modifications in VSG glycosylation and reduced virulence in Trypanosoma brucei

• DOI: 10.1111/mmi.12359
• Publication Date: 2013-08-08T15:22:02Z
• Authors: Víctor M. Castill...; Antonio E. Vidal; Luis M. Ruiz‐Pérez; Els J. M. Van Damme; Yasuhiro Igarashi; Jan Balzarini; Dolores González‐...
• Keywords: 0301 basic medicine; Glycosylation; Virulence; Cell Cycle; Trypanosoma brucei brucei; Mannosyltransferases; Trypanocidal Agents; Endocytosis; Cell Line; Mice, Inbred C57BL; Disease Models, Animal; Mice; 03 medical and health sciences; Mannose-Binding Lectins; Trypanosomiasis, African; Liliaceae; Animals; Humans; Variant Surface Glycoproteins, Trypanosoma

SummaryThe surface of Trypanosoma brucei is covered by a dense coat of glycosylphosphatidylinositol‐anchored glycoproteins. The major component is the variant surface glycoprotein (VSG) which is glycosylated by both paucimannose and oligomannose N‐glycans. Surface glycans are poorly accessible and killing mediated by peptide lectin–VSG complexes is hindered by active endocytosis. However, contrary to previous observations, here we show that high‐affinity carbohydrate binding agents bind to surface glycoproteins and abrogate growth of T. brucei bloodstream forms. Specifically, binding of the mannose‐specific Hippeastrum hybrid agglutinin (HHA) resulted in profound perturbations in endocytosis and parasite lysis. Prolonged exposure to HHA led to the loss of triantennary oligomannose structures in surface glycoproteins as a result of genetic rearrangements that abolished expression of the oligosaccharyltransferase TbSTT3B gene and yielded novel chimeric enzymes. Mutant parasites exhibited markedly reduced infectivity thus demonstrating the importance of specific glycosylation patterns in parasite virulence.