Nucleo‐cytoplasmic shuttling dynamics of the transcriptional regulators XYR1 and CRE1 under conditions of cellulase and xylanase gene expression in Trichoderma reesei
Transcription
Conidiation
DOI:
10.1111/mmi.12824
Publication Date:
2014-10-10T07:47:32Z
AUTHORS (4)
ABSTRACT
Trichoderma reesei is a model for investigating the regulation of (hemi-)cellulase gene expression. Cellulases are formed adaptively, and transcriptional activator XYR1 carbon catabolite repressor CRE1 main regulators their We quantified nucleo-cytoplasmic shuttling dynamics GFP-fusion proteins both transcription factors under cellulase xylanase inducing conditions, correlated nuclear presence/absence with changes. also compared subcellular localization in conidial germlings mature hyphae. show that expression requires de novo biosynthesis its simultaneous import, whereas repression regulated through preformed imported from cytoplasmic pool. Termination induction immediately stopped was accompanied by rapid degradation XYR1. In contrast, rapidly decreased upon glucose depletion, became recycled into cytoplasm. hyphae, nuclei containing activated were concentrated colony center, indicating this region synthesis transcription. found to be evenly distributed throughout entire mycelium. Taken together, our data revealed novel aspects dynamic spatial bias major regulator expression, XYR1, T. reesei.
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