Acetyl‐CoA synthetases of Saccharopolyspora erythraea are regulated by the nitrogen response regulator GlnR at both transcriptional and post‐translational levels

• DOI: 10.1111/mmi.13595
• Publication Date: 2016-12-17T17:27:34Z
• Authors: Di You; Miao‐Miao Wang; Bang‐Ce Ye
• Keywords: Response regulator

Summary Saccharopolyspora erythraea has three AMP‐forming acetyl‐CoA synthetases (Acs) encoded by acsA1 , acsA2 and acsA3 . In this work, we found that nitrogen response regulator GlnR can directly interact with the promoter regions of all genes activate their transcription in to availability. The typical GlnR‐binding boxes were identified regions. Moreover, activities Acs enzymes modulated reversible lysine acetylation (RLA) acetyltransferase AcuA NAD + ‐dependent deacetylase SrtN. Interestingly, controlled RLA activating expression acuA srtN A glnR ‐deleted mutant (Δ ) caused a growth defect 10 mM acetate minimal medium, condition under which function is critical control activity. Overexpression reversed Δ mutant. Total activity cell‐free extracts from strain had 4‐fold increase relative wildtype strain. Western Blotting showed vivo levels influenced availability lack These results demonstrated regulated at transcriptional post‐translational levels, mediated interplay between carbon metabolisms integrating signals modulate metabolism.