Rce1, a novel transcriptional repressor, regulates cellulase gene expression by antagonizing the transactivator Xyr1 in Trichoderma reesei

• DOI: 10.1111/mmi.13685
• Publication Date: 2017-04-05T18:12:03Z
• Authors: Yanli Cao; Fanglin Zheng; Lei Wang; Guolei Zhao; Guanjun Chen; Weixin Zhang; Weifeng Liu
• Keywords: Chromatin immunoprecipitation; Transcription

Summary Cellulase gene expression in the model cellulolytic fungus Trichoderma reesei is supposed to be controlled by an intricate regulatory network involving multiple transcription factors. Here, we identified a novel transcriptional repressor of cellulase expression, Rce1. Disruption rce1 not only facilitated induced genes but also led significant delay terminating induction process. However, Rce1 did participate Cre1‐mediated catabolite repression. Electrophoretic mobility shift (EMSA) and DNase I footprinting assays combination with chromatin immunoprecipitation (ChIP) demonstrated that could bind directly cbh1 (cellobiohydrolase 1‐encoding) promoter region containing cluster Xyr1 binding sites. Furthermore, competitive revealed antagonized from promoter. These results indicate interactions exist between variety factors ensure tight energy‐efficient regulation T. . This study provides important clues regarding increased production