The reverse gyrase TopR1 is responsible for the homeostatic control of DNA supercoiling in the hyperthermophilic archaeon Sulfolobus solfataricus

• DOI: 10.1111/mmi.14424
• Publication Date: 2019-11-12T12:51:16Z
• Authors: Mohea Couturier; Danièle Gadelle; Patrick Forterre; Marc Nadal; Florence Garnier
• Keywords: Sulfolobus solfataricus; Hyperthermophile; Replisome

Maintaining an appropriate DNA topology with DNA-based processes (DNA replication, transcription and recombination) is crucial for all three domains of life. In bacteria, the homeostatic regulation controlling supercoiling relies on antagonistic activities two topoisomerases, TopoI gyrase. hyperthermophilic crenarchaea, presence such a regulatory system suggested as TopoVI reverse gyrase, catalyze activities. To test this hypothesis, we estimated compared number that gyrases, TopR1 TopR2, in Sulfolobus solfataricus cells maintained either at 80 or 88°C, reciprocally shifted from one temperature to other. From only exhibiting significant quantitative variations response up- down-shifts. addition, corresponding intrinsic these topoisomerases were tested vitro both temperatures. Although modulates activities, sole topoisomerase able function high temperature. Altogether, results presented study demonstrate, first time, topological state crenarchaeon regulated via control, which mainly mediated by fine-tuning TopR1.