TbsP and TrmB jointly regulate gapII to influence cell development phenotypes in the archaeon Haloferax volcanii

• DOI: 10.1111/mmi.15225
• Publication Date: 2024-01-11T08:18:40Z
• Authors: Rylee K. Hackley; Sungmin Hwang; Jake T. Herb; Preeti Bhanap; Katie Lam; Angie Vreugdenhil; Cynthia L. Darnell; Mar Martinez Pastor; Johnathan H. Martin; Julie A. Maupin‐F...; Amy K. Schmid
• Keywords: Haloferax volcanii

Microbial cells must continually adapt their physiology in the face of changing environmental conditions. Archaea living extreme conditions, such as saturated salinity, represent important examples resilience. The model salt-loving organism Haloferax volcanii exhibits remarkable plasticity its morphology, biofilm formation, and motility response to variations nutrients cell density. However, mechanisms regulating these lifestyle transitions remain unclear. In prior research, we showed that transcriptional regulator, TrmB, maintains rod shape related species Halobacterium salinarum by activating expression enzyme-coding genes gluconeogenesis metabolic pathway. Hbt. salinarum, TrmB-dependent production glucose moieties is required for surface glycoprotein biogenesis. Here, use a combination genetics quantitative phenotyping assays demonstrate TrmB essential growth under gluconeogenic conditions Hfx. volcanii. ∆trmB strain rapidly accumulated suppressor mutations gene encoding novel which name trmB suppressor, or TbsP (a.k.a. "tablespoon"). adhesion abiotic surfaces (i.e., formation) wild-type morphology motility. We functional genomics promoter fusion characterize regulons controlled each TbsP, including joint regulation glucose-dependent transcription gapII, encodes an enzyme. conclude coregulate gluconeogenesis, with downstream impacts on