Protein phosphatase 2A regulatory subunits perform distinct functional roles in the maize pathogen Fusarium verticillioides

• DOI: 10.1111/mpp.12023
• Publication Date: 2013-03-04T16:14:15Z
• Authors: Joon‐Hee Shin; Jung‐Eun Kim; Martha Malapi‐Wight; Yoon‐E. Choi; Brian D. Shaw; Won‐Bo Shim
• Keywords: Fumonisin

Summary F usarium verticillioides is a pathogen of maize causing ear rot and stalk rot. The fungus also produces fumonisins, group mycotoxins linked to disorders in animals humans. A cluster genes, designated FUM plays key role the synthesis fumonisins. However, our understanding regulatory mechanism fumonisin biosynthesis still incomplete. We have demonstrated previously that C pp1, protein phosphatase type 2A ( PP2A ) catalytic subunit, negatively regulates production involved cell shape maintenance. In general, three subunits, structural , B make up heterotrimer complex perform functions. Significantly, we identified two subunits F. genome, P pr1 pr2, which are homologous S accharomyces cerevisiae dc55 R ts1, respectively. this study, hypothesized pr2 regulation and/or development generated series mutants determine functional pr2. PPR1 deletion strain (Δppr1) resulted drastic growth defects, but increased microconidia production. PPR2 mutant (Δppr2) showed elevated production, similar Δcpp1 strain. Germinating Δppr1 conidia formed abnormally swollen cells with central septation site, whereas Δppr2 early hyphal branching during germination. kernel assay were slow colonize kernels, probably result defects rather than virulence defect. Results from study suggest carry out distinct roles fungal development.