A two‐component histidine kinase Shk1 controls stress response, sclerotial formation and fungicide resistance in Sclerotinia sclerotiorum

• DOI: 10.1111/mpp.12041
• Publication Date: 2013-06-03T10:30:48Z
• Authors: Yabing Duan; Changyan Ge; Shengming Liu; Jianxin Wang; Mingguo Zhou
• Keywords: Sclerotinia Sclerotiorum; Osmotic shock

Summary Fungal histidine kinases ( HKs ) are involved in osmotic and oxidative stress responses, hyphal development, fungicide sensitivity virulence. Members of HK class III known to signal through the high‐osmolarity glycerol mitogen‐activated protein kinase HOG MAPK ). In this study, we characterized S hk1 gene SS1G _12694.3), which encodes a putative , from plant pathogen clerotinia sclerotiorum . Disruption resulted resistance phenylpyrrole dicarboximide fungicides increased hyperosmotic H 2 O ‐induced stress. The mutant showed significant reduction vegetative growth was unable produce sclerotia. Quantitative real‐time polymerase chain reaction qRT‐PCR determination assays that expression SsHOG1 (the last og pathway) accumulation were regulated by gene, but PAK (p21‐activated kinase) not. addition, no change All defects restored genetic complementation deletion with wild‐type gene. These findings indicate is differentiation, sclerotial formation, adaption stresses, fungicides, Taken together, our results demonstrate, for first time, role two‐component