The Tomato spotted wilt virus cell‐to‐cell movement protein (NSM) triggers a hypersensitive response in Sw‐5‐containing resistant tomato lines and in Nicotiana benthamiana transformed with the functional Sw‐5b resistance gene copy

• DOI: 10.1111/mpp.12144
• Publication Date: 2014-04-11T07:35:17Z
• Authors: Mariana Hallwass; Athos Silva de Ol...; Erico de Campos D...; Dick Lohuis; Leonardo Silva Bo...; Alice Kazuko Inou...; Renato O. Resende; Richard Kormelink
• Keywords: Movement protein

Although the Sw-5 gene cluster has been cloned, and Sw-5b identified as functional copy that confers resistance to Tomato spotted wilt virus (TSWV), its avirulence (Avr) determinant not date. Nicotiana tabacum 'SR1' plants transformed with a of are immune without producing clear visual response on challenge TSWV, whereas it is shown here N. benthamiana gives rapid conspicuous hypersensitive (HR). Using these plants, from all structural non-structural TSWV proteins tested, cell-to-cell movement protein (NSM ) was confirmed Avr using Potato X (PVX) replicon or non-replicative pEAQ-HT expression vector system. HR induced in Sw-5b-transgenic well resistant near-isogenic tomato lines after agroinfiltration resistance-inducing (RI) strain (BR-01), but NSM resistance-breaking (RB) (GRAU). This first biological demonstration Sw-5-mediated triggered by protein.