Analysis of gene expression changes in peach leaves in response to Plum pox virus infection using RNA‐Seq

• DOI: 10.1111/mpp.12169
• Publication Date: 2014-07-02T14:19:37Z
• Authors: Manuel Rubio; Luis Rodríguez‐Mo...; Ana Rosa Ballester; Manuel Castro de ...; Claudio Bonghi; Thierry Candresse; Pedro Martínez‐Gómez
• Keywords: 0301 basic medicine; 570; [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]; 610; PPV; plant-virus interaction; 3. Good health; 03 medical and health sciences; Plum Pox Virus; Gene Expression Regulation, Plant; Host-Pathogen Interactions; RNA-Seq; sharka disease; Prunus; Plant Diseases

Differences in gene expression were studied after Plum pox virus (PPV, sharka disease) infection peach GF305 leaves with and without symptoms using RNA-Seq. For each sample, more than 80% of 100-nucleotide paired-end (PE) Illumina reads aligned on the reference genome. In symptomatic a significant proportion mapped to PPV genomes (1.04% compared 0.00002% non-symptomatic leaves), allowing for ultra-deep assembly complete genome isolate used (9775 nucleotides, missing only 11 nucleotides at 5' end). addition, alternative splicing events detected 359 genes 12 990 single nucleotide polymorphisms (SNPs) identified, 425 which could be annotated. Gene ontology annotation revealed that high-ranking mRNA target associated are mainly related response biotic stimuli, lipid carbohydrate metabolism negative regulation catalytic activity. A greater number differentially expressed observed early asymptomatic phase comparison phase. These induction pathogen resistance, such as jasmonic acid, chitinases, cytokinin glucosyl transferases Lys-M proteins. Once had accumulated, overexpression Dicer protein 2a suggested silencing plant was suppressed by HCPro P1 results illustrate dynamic nature peach-PPV interaction transcriptome level confirm symptom is complex process can understood basis changes expression.