A pleiotropic drug resistance transporter is involved in reduced sensitivity to multiple fungicide classes in Sclerotinia homoeocarpa (F.T. Bennett)

• DOI: 10.1111/mpp.12174
• Publication Date: 2014-07-11T08:54:40Z
• Authors: Hyunkyu Sang; Jon Hulvey; James T. Popko; John Lopes; Aishwarya Swamina...; Taehyun Chang; Geunhwa Jung
• Keywords: 2. Zero hunger; Niacinamide; 0301 basic medicine; Sequence Analysis, RNA; Hydantoins; Biphenyl Compounds; Genetic Complementation Test; Membrane Transport Proteins; Saccharomyces cerevisiae; Triazoles; Aminoimidazole Carboxamide; Polymerase Chain Reaction; 3. Good health; Fungicides, Industrial; Fungal Proteins; 03 medical and health sciences; Ascomycota; Drug Resistance, Fungal; Gene Expression Regulation, Fungal; Linear Models; Transcriptome; Phylogeny; Disease Resistance; Plant Diseases

Summary Dollar spot, caused by S clerotinia homoeocarpa , is a prevalent turfgrass disease, and the fungus exhibits widespread fungicide resistance in N orth A merica. In previous study, an ABC ‐ G transporter, ShatrD was associated with practical field to demethylation inhibitor ( DMI ) fungicides. Mining of transporters, also known as pleiotropic drug PDR from RNA eq data gave assortment transcripts, several high sequence similarity functionally characterized transporters B otrytis cinerea others closest blastx hits Aspergillus Monilinia . addition another transporter showed significant over‐expression replicated data, collection field‐resistant isolates, measured quantitative polymerase chain reaction. These isolates reduced sensitivity unrelated classes. Using yeast complementation system, we sought test hypothesis that this effluxes well chemically The ShPDR1 cloned into Gal1 expression vector transformed deletion mutant, AD12345678 Complementation assays indicated complemented mutant presence propiconazole ), iprodione (dicarboximide) boscalid SDHI succinate dehydrogenase inhibitor). Our results indicate correlated fungicides dicarboximide findings highlight potential for eventual development multidrug phenotype pathogen. addition, study presents pipeline discovery validation genes using de novo next‐generation sequencing molecular biology techniques unsequenced plant pathogenic fungus.